In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector
- Freie Universität Berlin, Neurobiologie
Published 20 May. 2022 | License Creative Commons CC0 1.0 Public Domain Dedication
In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.
Keywords| Neuroscience | Insect | Apis mellifera | Honeybee | in-vivo electroporation | viral transduction | eGFP |
- In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector. Gérard Leboulle, Nora Gehne, Anja Froese, Randolf Menzel. Accepted for publication in PLoS ONE.
- DFG LE1809/1-1
- DFG LE1809/1-2