Dataset
Chronic in vivo calcium imaging dataset of neuronal populations in the auditory cortex of passively listening mice. Baseline and auditory cued fear conditioning cohorts
- 1JGU Mainz
- 2FIAS Frankfurt
DOI: 10.12751/g-node.trwj8c BROWSE REPOSITORY BROWSE ARCHIVE DOWNLOAD ARCHIVE (ZIP 261 GiB)
Published 07 Jan. 2022 | License Creative Commons CC0 1.0 Public Domain Dedication
Description
In this repository is a dataset of chronically imaged neuronal populations in the auditory cortex of mice being exposed to a set of short sound stimuli. The imaging was performed on four timepoints two days apart each over the course of seven days. Two different experimental conditions, one cohort was imaged under baseline conditions without any behavioral manipulations. The other cohort was imaged with an auditory cued fear conditioning session in between the second and third imaging time point. Image time series are two-channel images, channel one is histone fused mCherry (red fluorescent protein), H2B::mCherry. Channel two is GCaMP6m, green fluorescent genetically encoded calcium indicator. Additionally, we provide the information about auditory sensory stimulations in the file 'stimulation_info.zip'. Additionally, we provide the information about extracted rois after image processing in the file 'roi_coordinates.zip'. ROIs were aligned using a software package for image processing which can be downloaded from a public repository (DOI: 10.5281/zenodo.5822486). Further information about analysis of this dataset can be found in the corresponding published article.
Keywords
| Neuroscience | Auditory | Mouse | Two-Photon | Calcium Imaging | Chronic | Basal Conditions | Fear Conditioning | Learning |References
- Eppler, Jens-Bastian (2022) 2-photon data preprocessing pipeline (1.0). Zenodo. https://doi.org/10.5281/zenodo.5822486