Long-term in vivo application of a potassium channel-based optogenetic silencer in the healthy and epileptic mouse hippocampus
- 1Experimental Epilepsy Research, Department of Neurosurgery, Medical Center - University of Freiburg, Faculty of Medicine, 79106, Freiburg, Germany
- 2Experimental Biophysics, Institute of Biology, Humboldt University of Berlin, 10115 Berlin, Germany
Published 07 Dec. 2021 | License Creative Commons CC0 1.0 Public Domain Dedication
Here we provide the electrophysiological datasets and Python scripts, which underlie the conclusions in our paper about application of the novel potassium channel-based optogenetic silencer, PACK, in vivo (Kleis et al., 2021, preprint in BioRxiv and submitted publication in BMC Biology). We recorded the local field potentials (LFPs, resampled at 500 Hz, uploaded in .h5 format) with wire electrodes, which were chronically implanted into the hippocampus of freely behaving healthy or non-epileptic adult male mice. The six experimental groups are named in LFP_nomenclature_and_datasets.xlsx with further details in the published article. The Python scripts contain functions for calculating and plotting line length, power spectral density, and spectrograms.
Keywords| bPAC | cyclic AMP | Electrophysiology | Epilepsy | Hippocampus | Kainate | Neuroscience | Optogenetic inhibition | Potassium |
- Kleis, P., Paschen, E., Haeussler, U., Sierra, Y.A.B. and Haas, C.A., 2021. In vivo characterization and application of a novel potassium channel-based optogenetic silencer in the healthy and epileptic mouse hippocampus. bioRxiv.
- DFG HA 1443/11-1
- DFG SPP1926